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Life Science Optics

使用 Fura-2 测钙

FURA2-C calcium imaging set up荧光染料 Fura-2 的吸收光谱会根据荧光团分子附近存在的钙（Ca2+）浓度而显著变化。通过测量用两个不同的激发滤光片捕获的数字图像上的强度比，可以跟踪不同位置的钙浓度变化。

与软镀膜的滤光片比较， BrightLine® FURA2-C 滤光片组 提供 4 倍的亮度，还具有更高的信噪比，提供出色的高速成像性能。

此外，BrightLine® 滤光片组可实现饱和到自由信号强度的平衡，从而可以在不需对摄像机设置进行调整的情况下，进行准确的比率计算。

图 1. 高 (饱和 Ca2+) 浓度

FURA-2 成像

图 2. 低 (游离 Ca22+) 浓度

低钙的 FURA-2 成像

成功案例：

Nature: A gain-of-function TPC2 variant R210C increases affinity to PI(3,5)P2 and causes lysosome acidification and hypopigmentation

Published: 14 January 2023 - Nature.com - Nature Communication volume 14, P: 1-16

…Cells were alternatively excited at 340 and 380 nm, using a single-band multi-exciter filter set (Fura2-C-000, BrightLine®, Semrock), and emission was collected through a 409 nm edge single-edge dichroic beam splitter (BrightLine®, Semrock) and a 510/584 nm single-band emitter (BrightLine®, Semrock). ...