Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings. This 5-filter “Pinkel” triple-band set is designed for imaging of a sample that is simultaneously labeled with CFP, YFP, and mCherry, (or other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light sources compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a triple-band beamsplitter and a triple-band emitter (these can be offered assembled in a microscope cube), plus three “no burn-out” single-band exciters that are intended to be mounted in the LED based light source, or in an exciter filter wheel or slider. Interested in learning how this filter set will perform with your light source? Try Semrock’s SearchLight plotting & calculation tool.