021-52415599

BrightLine® full-multiband filter set, optimized for DAPI, FITC, TRITC, Cy5, & Cy7 and other like fluorophores, illuminated with LED-based light engines

Semrock部件编号: LED-DA/FI/TR/Cy5/Cy7-A 000

This Full-multiband penta-band set is designed for imaging DAPI, FITC, TRITC, Cy5 and Cy7 (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in penta-band performance, and has been optimized for popular LED-based light engines including 365 nm DAPI LEDs. This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a penta-band beamsplitter, emitter, and exciter filter, all with "no burn-out" performance.

比较产品

General Filter Specifications	Value
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications	Value
Scratch-Dig	60-40
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Dichroic Orientation	Reflective coating side should face toward light source and sample.
General Filter Specifications	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Specifications	Value
Physical Filter Specifications	Value

0 total selected Clear selection

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
Alexa Fluor® 405	401	422
BD Horizon V450	406	449
BFP (EBFP)	380	440
Cascade Blue™	401	419
CellTrace calcein violet	400	452
DAPI	359	461
DyLight 405	398	420
HiLyte Fluor™ 405	403	428
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
LysoSensor Blue	374	424
Marina Blue	363	461
Pacific Blue™	404	455
sgBFP™	387	451
SpectrumBlue	405	449
TagBFP	402	457
Vybrant DyeCycle Violet	369	437