021-52415599

BrightLine® full-multiband laser filter set, optimized for 405, 488, 555, & 640 nm laser sources

Semrock部件编号: LF405/488/555/640-A-000

This full-multiband set is optimized for laser excitation over four bands – utilizing a 375 or 405 nm laser source for DAPI, 488 nm laser source for GFP or FITC, a 555, 561 nm laser source for mCherry, and 635 nm and 640 nm laser source for Cy5. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio.This multiband filter set is recommended for LDI light sources.

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General Filter Specifications	Value
Optical Damage Rating	Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications	Value
Scratch-Dig	60-40
Exciter Emitter Orientation	Arrow on ring indicates preferred direction of propagation of light.
Blocking	BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Dichroic Orientation	Reflective coating side should face toward light source and sample.
General Filter Specifications	Value
Microscope Compatibility	BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Specifications	Value
Physical Filter Specifications	Value

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DAPI-5060C-OMF-ZERO

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DAPI-5060C-ZHE

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LED-CFP/YFP/mCherry-3X3M-A-ZHE

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SpAqua-C-NTE

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GFP-30LP-B-OFX

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Avant-YFP-ZHE-ZERO

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Optimized – Filter set is specifically optimized for that fluorophore.

Compatible – Should result in nearly ideal performance in most situations.

Acceptable – Actual performance is dependent on specific experimental conditions.

Fluorophore	Peak Excitation Wavelength(nm)	Peak Emission Wavelength(nm)
1,8-ANS	372	477
4-methylumbelliferone	357	441
7-hydroxy-4-methylcoumarin	360	442
Alexa Fluor® 405	401	422
AMCA (Aminomethylcoumarin)	350	448
ATTO 390	392	477
BD Horizon V450	406	449
BFP (EBFP)	380	440
Calcofluor White	351	444
Cascade Blue™	401	419
CellTrace calcein violet	400	452
DAPI	359	461
DiFMU-pH 9.0	358	450
DY-415	418	470
DyLight 405	398	420
ER-Tracker™ Blue-White DPX	372	557
HiLyte Fluor™ 405	403	428
Hoechst 33258	352	455
LIVE-DEAD Fixable Violet Dead Cell Stain	403	455
LysoSensor Blue	374	424
LysoTracker Blue	373	422
Marina Blue	363	461
Pacific Blue™	404	455
sgBFP™	387	451
Sirius	355	424
SpectrumBlue	405	449
TagBFP	402	457
Vybrant DyeCycle Violet	369	437