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BrightLine® Pinkel 1λ P-V RWE super-resolution laser filter set, optimized for 488 & 561 nm laser sources

BrightLine® Pinkel 1λ P-V RWE super-resolution laser filter set, optimized for 488 & 561 nm laser sources

BrightLine® Pinkel 1λ P-V RWE super-resolution laser filter set, optimized for 488 & 561 nm laser sources

Semrock部件编号: LF488/561-2X-C-000

Semrock

This “Pinkel” multiband set includes single-band excitation filters and a dual-band emission & dichroic filter. It is optimized for laser excitation utilizing a 473 or 488 nm laser source for GFP or FITC and a 559, 561.4 or 568.2 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination. Loose and cube mounted filter sets are certified to deliver dichroic flatness Looking for this filter set in one of the following cubes: NQF or LDMK? Our previous version is still available for those cube configurations LF488/561-2X-B

¥0.00

比较产品
Optical Specifications Value
Laser Wavelength 1 high  

491 nm, 568.2 nm
Laser Wavelength 1 low  

473 nm, 561.4 nm
Laser Wavelength 2  

488 nm
General Filter Specifications Value
Optical Damage Rating  

Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.
Physical Filter Specifications Value
Scratch-Dig  

60-40
Blocking  

BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.
Dichroic Orientation  

Reflective coating side should face toward light source and sample.
General Filter Specifications  

Value
Microscope Compatibility  

BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
Optical Specifications  

Value
Physical Filter Specifications  

Value

LF488/561-2X-C-NTE

比较产品
更多信息
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
Acridine Orange (+DNA) 502 526
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
AmCyan1 458 489
ATTO 488 500 525
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
Calcein 494 514
Calcium Green-1 507 529
CFP2 490 510
Cy2™ 492 507
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
DiO 475 500
Dronpa 502 516
DY-505-Phalloidin 503 530
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mHoneyDew 478 562
MitoTracker™ Green 490 512
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PicoGreen® 502 522
PKH67 488 500
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
ratiometric pHluorin pH5 500 509
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
sgGFP™ (super glow GFP) 472 506
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TO-PRO-1 515 531
TOTO-1 514 531
TurboGFP 482 503
Vybrant DyeCycle Green 506 534
wtGFP (wild type GFP, non-UV excitation) 474 509
YO-PRO-1 491 506
YOYO-1 491 508
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  • Image
  • SKU
  • Price
  • Optical Specifications
  • Angle of Incidence
  • Bandpass
  • Beamsplitter maximum thickness
  • Blocking
  • Blocking Band 1
  • Blocking Band 10
  • Blocking Band 11
  • Blocking Band 12
  • Blocking Band 13
  • Blocking Band 14
  • Blocking Band 15
  • Blocking Band 16
  • Blocking Band 17
  • Blocking Band 18
  • Blocking Band 2
  • Blocking Band 3
  • Blocking Band 4
  • Blocking Band 5
  • Blocking Band 6
  • Blocking Band 7
  • Blocking Band 8
  • Blocking Band 9
  • Blocking band 15
  • Center Wavelength 1
  • Center Wavelength 10
  • Center Wavelength 11
  • Center Wavelength 2
  • Center Wavelength 3
  • Center Wavelength 4
  • Center Wavelength 5
  • Center Wavelength 6
  • Center Wavelength 7
  • Center Wavelength 8
  • Center Wavelength 9
  • Clear Aperture
  • Common Fluorophore Pair to Split
  • Cutoff Transition Width
  • Cuton Transition Width
  • Dichroic Beamsplitters
  • Dichroic Flatness
  • Dichroic Orientation
  • Edge
  • Edge Steepness
  • Edge Wavelength 1
  • Edge Wavelength 2
  • Edge Wavelength 3
  • Edge Wavelength 4
  • Edge Wavelength 5
  • Exciter Emitter Orientation
  • F1' Error
  • Flatness
  • GMBW 1
  • General Filter Specifications
  • Group Delay Dispersion
  • Group Delay Dispersion 1
  • Group Delay Dispersion 2
  • Guaranteed Minimum Bandwidth 1
  • Guaranteed Minimum Bandwidth 2
  • Guaranteed Minimum Bandwidth 3
  • Guaranteed Minimum Bandwidth 4
  • Guaranteed Minimum Bandwidth 5
  • Guaranteed Minimum Bandwidth 9
  • Laser Wavelength 1
  • Laser Wavelength 1 high
  • Laser Wavelength 1 low
  • Laser Wavelength 2
  • Laser Wavelength 3
  • Laser Wavelength 4
  • Laser Wavelength 5
  • Laser Wavelengths 1
  • Laser Wavelengths 2
  • Laser Wavelengths 4
  • Maximum Reflected Laser Beam Diameter
  • MercuryLine
  • Microscope Compatibility
  • Nominal Flatness
  • Notch Bandwidth 1
  • Optical Damage Rating
  • Optical Specifications
  • Orientation
  • Physical Filter Specifications
  • Pulse Dispersion
  • Reflected Laser Wavelengths
  • Reflected Wavefront Error
  • Reflection Band 1
  • Reflection Band 2
  • Reflection Band 3
  • Reflection Band 4
  • Reflection Band 5
  • Special use
  • Steepness
  • Substrate Type
  • Transmission
  • Transmission Band 1
  • Transmission Band 10
  • Transmission Band 11
  • Transmission Band 2
  • Transmission Band 3
  • Transmission Band 4
  • Transmission Band 5
  • Transmission Band 6
  • Transmission Band 7
  • Transmission Band 8
  • Transmission Band 9
  • Transmission Ripple
  • Transmission Value 1
  • Transmitted Laser Wavelengths
  • Transmitted Wavefront Error
  • Transverse Tolerance
  • General Filter Specifications
  • Angle of Incidence
  • Cone Half-angle
  • Optical Damage Rating
  • Physical Filter Specifications
  • Angle of Incidence
  • Bandpass
  • Beamsplitter maximum thickness
  • Blocking
  • Blocking Band 1
  • Blocking Band 10
  • Blocking Band 11
  • Blocking Band 12
  • Blocking Band 13
  • Blocking Band 14
  • Blocking Band 15
  • Blocking Band 16
  • Blocking Band 17
  • Blocking Band 18
  • Blocking Band 2
  • Blocking Band 3
  • Blocking Band 4
  • Blocking Band 5
  • Blocking Band 6
  • Blocking Band 7
  • Blocking Band 8
  • Blocking Band 9
  • Blocking band 15
  • Center Wavelength 1
  • Center Wavelength 10
  • Center Wavelength 11
  • Center Wavelength 2
  • Center Wavelength 3
  • Center Wavelength 4
  • Center Wavelength 5
  • Center Wavelength 6
  • Center Wavelength 7
  • Center Wavelength 8
  • Center Wavelength 9
  • Clear Aperture
  • Common Fluorophore Pair to Split
  • Cutoff Transition Width
  • Cuton Transition Width
  • Dichroic Beamsplitters
  • Dichroic Flatness
  • Dichroic Orientation
  • Edge
  • Edge Steepness
  • Edge Wavelength 1
  • Edge Wavelength 2
  • Edge Wavelength 3
  • Edge Wavelength 4
  • Edge Wavelength 5
  • Exciter Emitter Orientation
  • F1' Error
  • Flatness
  • GMBW 1
  • General Filter Specifications
  • Group Delay Dispersion
  • Group Delay Dispersion 1
  • Group Delay Dispersion 2
  • Guaranteed Minimum Bandwidth 1
  • Guaranteed Minimum Bandwidth 2
  • Guaranteed Minimum Bandwidth 3
  • Guaranteed Minimum Bandwidth 4
  • Guaranteed Minimum Bandwidth 5
  • Guaranteed Minimum Bandwidth 9
  • Laser Wavelength 1
  • Laser Wavelength 1 high
  • Laser Wavelength 1 low
  • Laser Wavelength 2
  • Laser Wavelength 3
  • Laser Wavelength 4
  • Laser Wavelength 5
  • Laser Wavelengths 1
  • Laser Wavelengths 2
  • Laser Wavelengths 4
  • Maximum Reflected Laser Beam Diameter
  • MercuryLine
  • Microscope Compatibility
  • Nominal Flatness
  • Notch Bandwidth 1
  • Optical Damage Rating
  • Optical Specifications
  • Orientation
  • Physical Filter Specifications
  • Pulse Dispersion
  • Reflected Laser Wavelengths
  • Reflected Wavefront Error
  • Reflection Band 1
  • Reflection Band 2
  • Reflection Band 3
  • Reflection Band 4
  • Reflection Band 5
  • Special use
  • Steepness
  • Substrate Type
  • Transmission
  • Transmission Band 1
  • Transmission Band 10
  • Transmission Band 11
  • Transmission Band 2
  • Transmission Band 3
  • Transmission Band 4
  • Transmission Band 5
  • Transmission Band 6
  • Transmission Band 7
  • Transmission Band 8
  • Transmission Band 9
  • Transmission Ripple
  • Transmission Value 1
  • Transmitted Laser Wavelengths
  • Transmitted Wavefront Error
  • Transverse Tolerance
  • Transverse Tolerance
  • Clear Aperture
  • Scratch-Dig
  • Substrate Type
  • Orientation
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