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BrightLine® Sedat 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 561, & 635 nm laser sources

BrightLine® Sedat 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 561, & 635 nm laser sources

BrightLine® Sedat 1λ P-V RWE super-resolution laser filter set, optimized for 405, 488, 561, & 635 nm laser sources

Semrock部件编号: LF488/561-2X2M-C-NTE

Semrock

This "Sedat" multiband set includes single-band exciter and emission filters, and a dual-band dichroic. It is optimized for laser excitation utilizing a 473 or 488 nm laser source for GFP or FITC and a 559, 561.4 or 568.2 nm laser source for mCherry. This set provides high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The dichroic offers industry-leading 1λ P-V RWE for minimal focus shift and aberrations of the laser beam spot up to 10 mm diameter to enable popular imaging and Super-Resolution techniques including TIRF, PALM, STORM, and Structured-Illumination. Loose and cube mounted filter sets are certified to deliver dichroic flatness Looking for this filter set in one of the following cubes: NQF or LDMK? Our previous version is still available for those cube configurations LF488/561-2X2M-B

¥2,734.00 – ¥3,062.00

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Set Components

FF01-609/54-25

1

Each
  • Tavg > 93% 582 – 636 nm
  • FWHM of 60.6 nm

FL-004693

FF01-563/9-25

1

Each
  • Tavg > 93% 559 – 568 nm
  • FWHM of 14.5 nm

FL-004686

FF02-482/18-25

1

Each
  • Tavg > 93% 473 – 491 nm
  • FWHM of 24.4 nm

FL-004712

FF01-525/45-25

1

Each
  • Tavg > 93% 502.5 – 547.5 nm
  • FWHM of 50.6 nm

FL-004680

Di03-R488/561-t1-25x36

1

Each
  • Tavg > 93% 503.3 – 543 nm
  • Ravg > 90% 350 ­– 471 nm

FL-007037

Optical Specifications Value
Laser Wavelength 1 high  

491 nm, 568.2 nm
Laser Wavelength 1 low  

473 nm
Laser Wavelength 2  

488 nm
Laser Wavelength 3  

561.4 nm
Bandpass  

Single-band
Dichroic Beamsplitters  

Multiedge
Dichroic Flatness  

Super-resolution / TIRF
Edge Steepness  

Standard (for Raman)

LF488/561-2X2M-C-OFF

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更多信息
¥2,734.00 – ¥3,062.00
Optimized – Filter set is specifically optimized for that fluorophore.
Compatible – Should result in nearly ideal performance in most situations.
Acceptable – Actual performance is dependent on specific experimental conditions.
Fluorophore Peak Excitation Wavelength(nm) Peak Emission Wavelength(nm) Compatibility Display on Plot above
5-carboxy-2,7-dichlorofluorescein 500 525
5-FAM (5-carboxyfluorescein) 492 518
6-carboxyrhodamine 6G 525 555
Acridine Orange (+DNA) 502 526
Alexa Fluor® 430 431 541
Alexa Fluor® 488 499 520
Alexa Fluor® 500 503 525
AmCyan1 458 489
ATTO 488 500 525
ATTO 495 495 527
BCECF (pH 5.5) 481 518
BCECF (pH 9.0) 502 528
BODIPY FL 505 512
BODIPY FL-MeOH 502 511
Calcein 494 514
Calcium Green-1 507 529
CFP2 490 510
Cy2™ 492 507
CyQUANT GR-DNA 502 523
DAF-FM-NO 495 519
Dendra2 (Green) 491 507
DiA 457 586
DiO 475 500
Dronpa 502 516
DY-505-Phalloidin 503 530
ecliptic pHluorin pH5.5 473 507
Emerald 491 511
evoglow-Bs1 449 496
FITC (Fluorescein) 495 519
FlAsH-CCPFCC 511 530
Fluo-3 506 527
Fluo-4 494 516
Fluorescein dextran 501 524
Fluorescein-pH 8.0 489 517
Fluoro-Emerald 494 518
FluoSpheres Yellow-Green fluorescent microspheres 503 514
FM 1-43 473 579
GFP (EGFP) 489 511
Green 496 496 520
Green 500 501 524
HCS LipidTOX Green neutral lipid stain 498 507
HCS LipidTOX Green phospholipidosis 504 536
HiLyte Fluor™ 488 497 526
LIVE-DEAD Fixable Green Dead Cell Stain 498 525
LysoSensor Green 448 502
LysoTracker Green 501 509
LysoTracker Yellow HCK-123 488 565
Magnesium Green 507 531
mHoneyDew 478 562
MitoTracker™ Green 490 512
mWasabi 493 509
NBD-X (MeOH) 467 538
NeuroTrace 500/525 Green Fluorescent Nissl Stain 495 524
Oregon Green™ 488 498 526
Oregon Green™ 514 513 532
PicoGreen® 502 522
PKH67 488 500
Qdot® 525 Nanocrystals 300 525
Qdot® 545 Nanocrystals 300 543
ratiometric pHluorin pH5 500 509
Rhodamine 110 497 519
Rhodamine 123 507 529
Rhodamine Green 497 523
Rhodol Green 497 524
sgGFP™ (super glow GFP) 472 506
Sodium Green 507 532
SpectrumGreen 497 538
SYBR Gold nucleic acid gel stain-DNA 496 539
SYBR Green I nucleic acid gel stain-DNA 498 522
SYBR Safe DNA gel stain-DNA 509 526
SYTO 11 506 525
SYTO 13 448 506
SYTO 16 489 520
SYTO 9 483 500
SYTO RNASelect geen fluorescent cell stain 503 527
SYTOX Green-DNA 504 524
TO-PRO-1 515 531
TOTO-1 514 531
TurboGFP 482 503
Vybrant DyeCycle Green 506 534
wtGFP (wild type GFP, non-UV excitation) 474 509
YO-PRO-1 491 506
YOYO-1 491 508
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  • Image
  • SKU
  • Price
  • Optical Specifications
  • Angle of Incidence
  • Bandpass
  • Beamsplitter maximum thickness
  • Blocking
  • Blocking Band 1
  • Blocking Band 10
  • Blocking Band 11
  • Blocking Band 12
  • Blocking Band 13
  • Blocking Band 14
  • Blocking Band 15
  • Blocking Band 16
  • Blocking Band 17
  • Blocking Band 18
  • Blocking Band 2
  • Blocking Band 3
  • Blocking Band 4
  • Blocking Band 5
  • Blocking Band 6
  • Blocking Band 7
  • Blocking Band 8
  • Blocking Band 9
  • Blocking band 15
  • Center Wavelength 1
  • Center Wavelength 10
  • Center Wavelength 11
  • Center Wavelength 2
  • Center Wavelength 3
  • Center Wavelength 4
  • Center Wavelength 5
  • Center Wavelength 6
  • Center Wavelength 7
  • Center Wavelength 8
  • Center Wavelength 9
  • Clear Aperture
  • Common Fluorophore Pair to Split
  • Cutoff Transition Width
  • Cuton Transition Width
  • Dichroic Beamsplitters
  • Dichroic Flatness
  • Dichroic Orientation
  • Edge
  • Edge Steepness
  • Edge Wavelength 1
  • Edge Wavelength 2
  • Edge Wavelength 3
  • Edge Wavelength 4
  • Edge Wavelength 5
  • Exciter Emitter Orientation
  • F1' Error
  • Flatness
  • GMBW 1
  • General Filter Specifications
  • Group Delay Dispersion
  • Group Delay Dispersion 1
  • Group Delay Dispersion 2
  • Guaranteed Minimum Bandwidth 1
  • Guaranteed Minimum Bandwidth 2
  • Guaranteed Minimum Bandwidth 3
  • Guaranteed Minimum Bandwidth 4
  • Guaranteed Minimum Bandwidth 5
  • Guaranteed Minimum Bandwidth 9
  • Laser Wavelength 1
  • Laser Wavelength 1 high
  • Laser Wavelength 1 low
  • Laser Wavelength 2
  • Laser Wavelength 3
  • Laser Wavelength 4
  • Laser Wavelength 5
  • Laser Wavelengths 1
  • Laser Wavelengths 2
  • Laser Wavelengths 4
  • Maximum Reflected Laser Beam Diameter
  • MercuryLine
  • Microscope Compatibility
  • Nominal Flatness
  • Notch Bandwidth 1
  • Optical Damage Rating
  • Optical Specifications
  • Orientation
  • Physical Filter Specifications
  • Pulse Dispersion
  • Reflected Laser Wavelengths
  • Reflected Wavefront Error
  • Reflection Band 1
  • Reflection Band 2
  • Reflection Band 3
  • Reflection Band 4
  • Reflection Band 5
  • Special use
  • Steepness
  • Substrate Type
  • Transmission
  • Transmission Band 1
  • Transmission Band 10
  • Transmission Band 11
  • Transmission Band 2
  • Transmission Band 3
  • Transmission Band 4
  • Transmission Band 5
  • Transmission Band 6
  • Transmission Band 7
  • Transmission Band 8
  • Transmission Band 9
  • Transmission Ripple
  • Transmission Value 1
  • Transmitted Laser Wavelengths
  • Transmitted Wavefront Error
  • Transverse Tolerance
  • General Filter Specifications
  • Angle of Incidence
  • Cone Half-angle
  • Optical Damage Rating
  • Physical Filter Specifications
  • Angle of Incidence
  • Bandpass
  • Beamsplitter maximum thickness
  • Blocking
  • Blocking Band 1
  • Blocking Band 10
  • Blocking Band 11
  • Blocking Band 12
  • Blocking Band 13
  • Blocking Band 14
  • Blocking Band 15
  • Blocking Band 16
  • Blocking Band 17
  • Blocking Band 18
  • Blocking Band 2
  • Blocking Band 3
  • Blocking Band 4
  • Blocking Band 5
  • Blocking Band 6
  • Blocking Band 7
  • Blocking Band 8
  • Blocking Band 9
  • Blocking band 15
  • Center Wavelength 1
  • Center Wavelength 10
  • Center Wavelength 11
  • Center Wavelength 2
  • Center Wavelength 3
  • Center Wavelength 4
  • Center Wavelength 5
  • Center Wavelength 6
  • Center Wavelength 7
  • Center Wavelength 8
  • Center Wavelength 9
  • Clear Aperture
  • Common Fluorophore Pair to Split
  • Cutoff Transition Width
  • Cuton Transition Width
  • Dichroic Beamsplitters
  • Dichroic Flatness
  • Dichroic Orientation
  • Edge
  • Edge Steepness
  • Edge Wavelength 1
  • Edge Wavelength 2
  • Edge Wavelength 3
  • Edge Wavelength 4
  • Edge Wavelength 5
  • Exciter Emitter Orientation
  • F1' Error
  • Flatness
  • GMBW 1
  • General Filter Specifications
  • Group Delay Dispersion
  • Group Delay Dispersion 1
  • Group Delay Dispersion 2
  • Guaranteed Minimum Bandwidth 1
  • Guaranteed Minimum Bandwidth 2
  • Guaranteed Minimum Bandwidth 3
  • Guaranteed Minimum Bandwidth 4
  • Guaranteed Minimum Bandwidth 5
  • Guaranteed Minimum Bandwidth 9
  • Laser Wavelength 1
  • Laser Wavelength 1 high
  • Laser Wavelength 1 low
  • Laser Wavelength 2
  • Laser Wavelength 3
  • Laser Wavelength 4
  • Laser Wavelength 5
  • Laser Wavelengths 1
  • Laser Wavelengths 2
  • Laser Wavelengths 4
  • Maximum Reflected Laser Beam Diameter
  • MercuryLine
  • Microscope Compatibility
  • Nominal Flatness
  • Notch Bandwidth 1
  • Optical Damage Rating
  • Optical Specifications
  • Orientation
  • Physical Filter Specifications
  • Pulse Dispersion
  • Reflected Laser Wavelengths
  • Reflected Wavefront Error
  • Reflection Band 1
  • Reflection Band 2
  • Reflection Band 3
  • Reflection Band 4
  • Reflection Band 5
  • Special use
  • Steepness
  • Substrate Type
  • Transmission
  • Transmission Band 1
  • Transmission Band 10
  • Transmission Band 11
  • Transmission Band 2
  • Transmission Band 3
  • Transmission Band 4
  • Transmission Band 5
  • Transmission Band 6
  • Transmission Band 7
  • Transmission Band 8
  • Transmission Band 9
  • Transmission Ripple
  • Transmission Value 1
  • Transmitted Laser Wavelengths
  • Transmitted Wavefront Error
  • Transverse Tolerance
  • Transverse Tolerance
  • Clear Aperture
  • Scratch-Dig
  • Substrate Type
  • Orientation
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